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A5 9: 30 BRAIN COOLING: AN EFFECTIVE INTERVENTION FOR INDIVIDUALS WITH SUSPECTED STROKE? Thornhill, Jim & Ramsden * , Viv, Dept's of Physiology & Family Medicine, College of Medicine, Univ. of Sask. S7N 5E5 Mild hypothermia evoked to focally or globally ischemic animals provides long term protection from the insult. Translating this technique to individuals suspected of stroke is challenging due to concerns of associated increases in energy metabolism and blood pressure, rebound shivering and coagulation compromise as well as operational concerns of implementing this protocol with any individual regardless of the site of the suspected stroke. Utilizing a non-invasive strategy to selectively cool the brain to minimize the unwanted side effects, a cooling collar and temperature monitoring device have been patented US # 6, 682, 552 ; for individuals with suspected stroke in the pre-hospital setting home, ambulance ; which facilitates cooling of carotid blood entering the brain. Both animal rats ; and human healthy volunteers ; experiments were conducted to determine the appropriate duration and severity of carotid cooling using pre-cooled ingots ; that would be required to decrease brain temperature by 1 C. Male and female Long Evans rats ~250g, n 14 ; , anesthetized with halothane 4% induction, 1% maintenance ; and maintained at 37C via servo controlled heating blanket ; had brain thalamic ; temperatures from both hemispheres via implanted thermistors ; measured before, during and after carotid cooling. Carotid cooling consisted of placing 21 C or pre-cooled 1-4C ; aluminum ingots of varying size 24-65g ; underneath the rat's neck for 45 minutes. Only slight decreases in brain temperature occurred after carotid cooling, from respective pre-cooling control temperatures. Rebound increases in brain temperature were seen after the cessation of carotid cooling. Human experiments with male and female healthy volunteers n 40 ; were also conducted to determine differences in core oral, tympanic, axilla ; and surface neck, temporal ; temperatures and of other vital signs in resting subjects over 20 minutes kept in a room with an average temperature of 23.6 C. A survey questionnaire was completed by each individual to assess predisposing variables e.g. smoking ; that may contribute to the core temperatures determined. Neck surface temperatures of resting volunteers were 1.5-4.0C below core temperatures in this control study. These animal and human Phase 1 ; experiments provided insights as to the challenges in adequately cooling carotid blood for reducing core brain ; temperature of suspected stroke patients using the cooling collar device. Supported by the CIHR and the Industry Liaison Office of the University of Saskatchewan CPS Winter Meeting 2005 2. 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ABSTRACT ECG pattern of dog. However, at 10 and 20 mg kg showed decreased heart rate and prolonged P-R, P-P, R-R and T-P intervals. CPF at 5 mg kg decreased both the rate and depth of respiration in dog and at 10 and 20 mg kg produced transient respiratory apnoea which could not be blocked by atropine. 308. A CARDIOACTIVE FACTOR FROM INDIAN KING COBRA OPHIOPHAGUS HANNAH ; VENOM DASGUPTA S. C. AND GOMES A. Lab of Toxinology and Exp Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P Road, Calcutta .C. 700 009, India King Cobra Ophiophagus hannah ; is the largest venomous land snake available in the north-eastern hilly region of the Indian subcontinent. Envenomation due to this snake is not very common but inevitably fatal. No antiserum exist against this venom. Practically no information is available on its venom venom constituents. The present investigation was an effort to identify a cardioactive factor present in the King Cobra venom. The cardio active factor CAF ; was isolated and purified by TLC using F254 silica gel coated glass plates. The solvent system used was isopropanol and 0.IN HCI 7: 3 ; . When observed under UV rays 254 nm ; the compound gave a brown fluorescence with a ref value of 0.38. This compound showed no peaks when scanned at 200-400 nm. NMR spectroscopy revealed that it contain CH3 group of long chain compound, molecular weight of which was found to be 500 dalton. CAF was found to be a non protein, non lethal compound and did not exhibit any effect on rat and guinea-pig blood pressure and respiration. CAF failed to show any response on rabbit aortic strip and did not show any neurotoxic activity tested on isolated RPND and CBC. CAF at lower dose produced a significant increase of heart rate and amplitude of isolated guinea-pig and toad heart, but at higher dose it showed inhibitory effect. On isolated arrhythmic heart and auricle of toad and guinea-pig induced by Acetylcholine and Aconitine ; CAF restored the heart rate and amplitude of contraction. Further information on the chemical characterization are warranted so that CAF could be used as a cardioactive agent. 309. PATHOPHYSIOLOGICAL EFFECTS OF JUVENILE COBRA VENOM Naja Kauothia ; ON EXPERIMENTAL ANIMAL GOMES A. AND DASGUPTA S. C. Lab of Toxinology and Exp Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C. Road Calcutta 700 009, India Snakebite, a major sociomedical problem of tropical countries. In India, a large number of people suffer and die each year due to snake envenomation. In most of the cases, the snake is difficult to identify specifically the variety, sex, age adult or juvenile ; . Plenty of information is available on the adult snake venoms. But practically no information is available on the Juvenile snake venom. Folklore information suggest that juvenile snake venom is more toxic than the adult snake. The present study is perhaps the first investigation on the Indian baby cobra Naja Kaouthia ; venom on experimental animals. Boby cobra 25 days old ; venom BCV ; was collected from Calcutta snake park.Yield of the venom was 11.5 + 1.18 mg per snake. Protein content of cobra venom was found to be 89.5 + 1.75 gm%. LD50 in male albino mice was.
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Are also using self-medication products. Additionally, promotional messages through the media and the Internet tend to convey a feeling of confidence in the safety of the product and often give the impression that self-medication products are just another consumer article. In other cases, excessive or non-medical use may be a problem. Reports have been received of OTC medicines being misused by drug addicts 2 ; and, according to a recent study in Northern Ireland, pharmacists admit that OTC drugs may be used in this way 3 ; . There are several critical issues involved before deciding if drugs should be authorized for selfmedication. First and foremost, is the principle that no drug is absolutely safe -- prescription drugs remain potent medications. Self-medication is, in the majority of cases, applied without medical supervision and, to a certain extent, is an uncharted area with regard to interactions, pregnancy, lactation, use in children and the elderly, driving, working conditions, alcohol, or food compared to the more controlled prescription-only environment. In many countries, the possibility of reporting adverse drug reactions ADR ; to self-medication products is not available since many conventional ADR reporting schemes operate through health care professionals. Only in a small number of countries with highly developed ADR systems are patients and consumers able to report ADRs directly to the authorities or through pharmacies. Moreover, clinical trial data for prescription use may not necessarily be valid for self-medication. This situation is beginning to improve within some countries that now demand OTC-environment studies to be undertaken before registration. Special mention should be made of the heavy reliance placed on OTC analgesics. These have long been associated with chronic renal failure. Many earlier reports implicated phenacetin-containing analgesics as the risk factor. Since the early 1980s, several case-control studies have reported associations between chronic renal failure and use of other forms of analgesics, including paracetamol, aspirin, and other nonsteroidal anti-inflammatory drugs NSAIDs ; . Although findings from these studies should be interpreted with caution, the use of OTC analgesics is widespread and the potential impact of these drugs on the development of chronic renal failure may be significant 4 ; . Furthermore, the consumer may be unaware that several products with different brand names and for different indications may contain the same active ingredient. Consumers need independent information to ensure the safe, effective and rational use of drugs in.
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Phone: 07 ; 3277 2588 Fax: 07 ; 3277 2099 windsory windsorygroup .au windsorygroup .au q Australian Woollen Products Blankets, Pillows, Carpets. Technology. The issue of differential prices with benefit from differential prices, we are not against differential prices, we could not be against that concept if it represents better access to pharmaceuticals. We are absolutely in favour of public private partnerships. I don't think it falls in the usual category of PPPs but very recently the Brazilian Government and the NGO, Medecins Sans Frontieres, has established an agreement to provide drugs to South Africa on a non-commercial basis. I cannot say that we are running away from every possible discussion and we will definitely not refrain from addressing the impact that patents have on access to medications. Regarding Dr Noehrenberg's point, I cannot understand the problem with advocating transfer of technology. That is the objective of the TRIPS Agreement. I don't understand your objection to that point. If we should take away that element from the TRIPS Agreement, I do not see what the purpose of the TRIPS Agreement would be for developing countries. It is better not to have the Agreement if we do not have that element which is the minimal we can expect for developing countries. Regarding the preservation of the right to copy in Brazil, as you know, what we have in terms of the existing, the strongest pharmaceutical industry established in Brazil, is exactly the international pharmaceutical industry. What we have as domestic, as indigenous, pharmaceutical industry is very small. We are not India. India does have an extremely wealthy and rich genetics pharmaceutical company. I wish we could have the same strength in terms of being prepared to produce generics but the reality is that we are not in the same position. What we have has been absorbed by the multinational industry so that's the reality and I find it contradictory to hear from a representative of Pharma that Brazil is applying an industrial policy in Brazil. It must be only for the benefit of the international pharmaceutical company and we do provide even tax incentives for the pharmaceutical company so what are we complaining about. Jeffrey Kemprecos: Merck and Co. Inc. First of all, Francisco is a very skilled and well-briefed negotiator and wish he could work for the Americans. One of the ways you would solve the ambiguities in the TRIPS Agreement would be to have, for instance, dispute settlement and, of course, Brazil and other countries have tried to thwart dispute settlements or place an indefinite moratoria on them. That inhibits the ability for the WTO member states to work out how you could solve the ambiguities. These extra agreement declarations we get seem to be the preferred route. I think Brazil has benefited enormously from its patent law and the change in the overall commercial environment there since 1996. I don't have the precise figures, but its to the tune of hundreds of millions of dollars in new investment from the multinationals and that clearly represents technology transfer. So I would ask Francisco, well how much is enough for Brazil. You have mentioned technology transfer several times. Brazil in the mid 90s anyway was doing many things to not only encourage our investment but serious investment internally from a budding biotech sector. Which brings me to my last point. It will be interesting to see Francisco in Geneva one day when Mexico is issuing a compulsory license for one of the biotech products coming out of Sao Paulo and I think these interests shift back and forth and none of them appears to be set in concrete, not that I want that to happen having lived through it in Brazil. It is not the most favourable investment or commercial environment when one negotiates with what is, in fact, a gun to the head. The New Yorker had an article in midDecember on India and I invite people to read this because it really strips away much. 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